How does she know Jaheira's fertile?
Tempest nailed it on the head. Isobel doesn't know Jaheira's fertile, but has no reason to think otherwise. She's never seen a half-elf until she came to Amn, but has read enough and heard enough stories to know half-elves generally aren't infertile, subject to genetic disorders, vulnerable to disease, or any of the other usual problems associated with successful crossbreeds between different species. Isobel does bring a degree of scientific learning and awareness that's very rare anywhere in the Forgotten Realms outside of a very few places, and Isobel is from one of those places. I would like to emphasize that her Intelligence score is a little misleading: Isobel is educated as well as intelligent.
I can nonetheless see Jaheira attempting to regain control of the conversation by pointing that out as an assumption.
By the way, congratulations and please consult this before making any important decisions. ^.^